August 31, 2022 – With the World Health Organization declaring the monkeypox virus (MPXV) a Public Health Emergency of International Concern, and more than 47,000 total cases across 99 countries reported to date and growing, Integrated DNA Technologies (IDT) is supporting researchers conducting monkeypox surveillance with a new next generation sequencing (NGS) solution. Designed as part of IDT’s NGS Tech Access, the program is intended to accelerate innovation by enabling access to the most advanced research tools that are still in development.
The xGen™ Monkeypox Virus Amplicon Panel is a complete NGS library preparation solution that supports the genotyping of MPXV within a sample. Leveraging IDT’s well-established NGS amplicon technology, the single-tube workflow creates overlapping amplicons, goes from sample to sequencer in 2.5 hours with up to 1,536 unique dual indexing (UDIs) for multiplexed sequencing, and helps pinpoint evolving epidemiological patterns. Orders can now be placed globally here.
The infectious disease monitoring and surveillance field continues to face unprecedented pressure for accuracy and increased speed. IDT’s xGen Monkeypox Virus Amplicon Panel is a single-tube workflow that generates contiguous tiling of overlapping amplicons. Super amplicons enable novel variant discovery by maintaining genomic coverage even if a novel variant occurs under a primer binding site, which may disrupt a primer’s ability to bind. IDT tested the panel with commercially available monkeypox DNA and demonstrated coverage of positions 6760–190,905 (inverted-terminal repeats (ITRs) not included). Data can be generated from extracted viral DNA following a 2.5-hour workflow from viral DNA to libraries. The xGen Monkeypox Viral Amplicon Panel is also compatible with IDT Normalase™ technology.